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kiaa1429 antibody (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc kiaa1429 antibody
Kiaa1429 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/kiaa1429+antibody/pm41826832-130-16-20
Average 86 stars, based on 1 article reviews

kiaa1429 antibody - by Bioz Stars, 2026-10

86/100 stars

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Article Title: USP7 Drives Atherosclerosis by Promoting Ferroptosis in Vascular Endothelial Cells via the KIAA1429/NEAT1/CTCF Axis.
Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS dodecyl sulfate polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes (88518, Thermo Fisher Scientific). .. Membranes were blocked with 5% non-fat milk (A600669, Sangon Biotech) for 2 hours at room temperature, then incubated with primary antibodies overnight at 4°C: USP7 antibody (1:500, ab4080, Abcam, Cambridge, MA, USA), KIAA1429 antibody (1:1000, 88358, Cell Signaling Technology, CST, Danvers, MA, USA), GPX4 antibody (1:1000, ab125066, Abcam), SLC7A11 antibody (1:1000, ab307601, Abcam), YTHDF1 antibody (1:1000, ab220162, Abcam), CTCF antibody (1:1000, ab128873, Abcam), and β-actin antibody (1:1000, ab8227, Abcam). .. After washing three times (10 minutes each) with 1×TBST buffer (T1085, Solarbio), membranes were incubated with secondary antibody Goat Anti-Rabbit immunoglobulin G (IgG) H&L (HRP) (1:2000, ab205718, Abcam) for 2 hours at room temperature.

Immunoprecipitation:

Article Title: USP7 Drives Atherosclerosis by Promoting Ferroptosis in Vascular Endothelial Cells via the KIAA1429/NEAT1/CTCF Axis.
Article Snippet: .. Lysates were boiled for 10 minutes, renatured in wash buffer, and subjected to immunoprecipitation experiments with KIAA1429 antibody (1:50, 88358, CST) or IgG antibody (1:25, ab6715, Abcam). .. Ubiquitination levels were detected by Western blot using ubiquitin antibody (1:1000, ab134953, Abcam).

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Comprehensive analysis of the KIAA1429 in the TCGA and GEO database (A) The expression of KIAA1429 in the TCGA-KIRCC database (tumor samples n = 523, normal samples n = 100). (B–D) The expression levels of KIAA1429 in ccRCC tissues and adjacent normal tissues (23 pairs of paired samples) in (B) GSE36895 dataset, (C) GSE53757 dataset (72 pairs of paired samples), and (D) GSE40435 dataset (101 pairs of paired samples). (E) The GEPIA database analyzed the expression of KIAA1429 in patients with ccRCC of different clinical stages. (F and G) The GEPIA database analyzed the effect of KIAA1429 mRNA expression on the OS and DFS of patients with ccRCC. Data are presented as the means ± SEM. ∗ p < 0.05, ∗∗∗ p < 0.001 by Student’s two-tailed t test.

Journal: iScience

Article Title: KIAA1429 promotes clear cell renal cell carcinoma progression by regulating MYC mRNA stability

doi: 10.1016/j.isci.2026.115198

Figure Lengend Snippet: Comprehensive analysis of the KIAA1429 in the TCGA and GEO database (A) The expression of KIAA1429 in the TCGA-KIRCC database (tumor samples n = 523, normal samples n = 100). (B–D) The expression levels of KIAA1429 in ccRCC tissues and adjacent normal tissues (23 pairs of paired samples) in (B) GSE36895 dataset, (C) GSE53757 dataset (72 pairs of paired samples), and (D) GSE40435 dataset (101 pairs of paired samples). (E) The GEPIA database analyzed the expression of KIAA1429 in patients with ccRCC of different clinical stages. (F and G) The GEPIA database analyzed the effect of KIAA1429 mRNA expression on the OS and DFS of patients with ccRCC. Data are presented as the means ± SEM. ∗ p < 0.05, ∗∗∗ p < 0.001 by Student’s two-tailed t test.

Article Snippet: KIAA1429 Rabbit Polyclonal antibody , Proteintech , Cat#24761-1-AP.

Techniques: Expressing, Two Tailed Test

KIAA1429 mRNA and protein expression was significantly upregulated in ccRCC tissues and cell lines and its relationship to clinicopathological parameters (A and B) The protein and mRNA levels of KIAA1429 in ccRCC and normal kidney tissues. (C and D) KIAA1429 mRNA and protein expression in ccRCC cell lines and normal epithelium cell of renal tubule (HK2) cells. (E and F) The expression level of KIAA1429 in ccRCC tissue and surrounding normal renal tissue was detected using IHC. Scale bars, 100 μm. (G) The expression level of KIAA1429 in patients with ccRCC with different clinical stages and Fuhrman’s grade. (H) Effect of KIAA1429 expression on the overall survival of patients with ccRCC. T, tumor; N, normal. Data are presented as the means ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 by Student’s two-tailed t test.

Journal: iScience

Article Title: KIAA1429 promotes clear cell renal cell carcinoma progression by regulating MYC mRNA stability

doi: 10.1016/j.isci.2026.115198

Figure Lengend Snippet: KIAA1429 mRNA and protein expression was significantly upregulated in ccRCC tissues and cell lines and its relationship to clinicopathological parameters (A and B) The protein and mRNA levels of KIAA1429 in ccRCC and normal kidney tissues. (C and D) KIAA1429 mRNA and protein expression in ccRCC cell lines and normal epithelium cell of renal tubule (HK2) cells. (E and F) The expression level of KIAA1429 in ccRCC tissue and surrounding normal renal tissue was detected using IHC. Scale bars, 100 μm. (G) The expression level of KIAA1429 in patients with ccRCC with different clinical stages and Fuhrman’s grade. (H) Effect of KIAA1429 expression on the overall survival of patients with ccRCC. T, tumor; N, normal. Data are presented as the means ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 by Student’s two-tailed t test.

Article Snippet: KIAA1429 Rabbit Polyclonal antibody , Proteintech , Cat#24761-1-AP.

Techniques: Expressing, Two Tailed Test

KIAA1429 promoted ccRCC cell proliferation in vitro (A) Knockdown efficiencies of KIAA1429 mRNA in ACHN and Caki-1 cells were detected using RT-qPCR; KIAA1429 protein expression decreased significantly in groups of shKIAA1429 in western blot analysis. (B and C) The effect of KIAA1429 knockdown on the proliferation of ccRCC cells was detected using a CCK-8 assay (B) and colony formation assay (C). (D) The effect of KIAA1429 on the cell cycle of ccRCC cells was detected using flow cytometry. Data are presented as the means ± SEM ∗ p < 0.05, ∗∗ p < 0.01 by Student’s two-tailed t test.

Journal: iScience

Article Title: KIAA1429 promotes clear cell renal cell carcinoma progression by regulating MYC mRNA stability

doi: 10.1016/j.isci.2026.115198

Figure Lengend Snippet: KIAA1429 promoted ccRCC cell proliferation in vitro (A) Knockdown efficiencies of KIAA1429 mRNA in ACHN and Caki-1 cells were detected using RT-qPCR; KIAA1429 protein expression decreased significantly in groups of shKIAA1429 in western blot analysis. (B and C) The effect of KIAA1429 knockdown on the proliferation of ccRCC cells was detected using a CCK-8 assay (B) and colony formation assay (C). (D) The effect of KIAA1429 on the cell cycle of ccRCC cells was detected using flow cytometry. Data are presented as the means ± SEM ∗ p < 0.05, ∗∗ p < 0.01 by Student’s two-tailed t test.

Article Snippet: KIAA1429 Rabbit Polyclonal antibody , Proteintech , Cat#24761-1-AP.

Techniques: In Vitro, Knockdown, Quantitative RT-PCR, Expressing, Western Blot, CCK-8 Assay, Colony Assay, Flow Cytometry, Two Tailed Test

KIAA1429 promoted ccRCC cell migration and invasion in vitro (A and B) The effect of KIAA1429 knockdown on the migration and invasion of Caki-1 and ACHN cells were detected using a cell wound-healing assay (A) and transwell assay (B). (C) Western blot analysis of vimentin, N-cadherin, and E-cadherin protein expression in Caki-1 and ACHN cells with KIAA1429 knockdown. Data are presented as the means ± SEM. ∗ p < 0.05 by Student’s two-tailed t test.

Journal: iScience

Article Title: KIAA1429 promotes clear cell renal cell carcinoma progression by regulating MYC mRNA stability

doi: 10.1016/j.isci.2026.115198

Figure Lengend Snippet: KIAA1429 promoted ccRCC cell migration and invasion in vitro (A and B) The effect of KIAA1429 knockdown on the migration and invasion of Caki-1 and ACHN cells were detected using a cell wound-healing assay (A) and transwell assay (B). (C) Western blot analysis of vimentin, N-cadherin, and E-cadherin protein expression in Caki-1 and ACHN cells with KIAA1429 knockdown. Data are presented as the means ± SEM. ∗ p < 0.05 by Student’s two-tailed t test.

Article Snippet: KIAA1429 Rabbit Polyclonal antibody , Proteintech , Cat#24761-1-AP.

Techniques: Migration, In Vitro, Knockdown, Wound Healing Assay, Transwell Assay, Western Blot, Expressing, Two Tailed Test

KIAA1429 promoted tumorigenesis of ccRCC cells in vivo (A) Subcutaneous tumor model of ACHN cells with KIAA1429 knockdown. KIAA1429 knockdown led to decreased tumor volume. (B and C) The changes in volume and weight were measured at the indicated weeks after mice were transplanted with the relevant cells. (D) IHC of vimentin, Ki-67, and E-cadherin protein expression in tumors with KIAA1429 knockdown. Data are presented as the means ± SEM. ∗ p < 0.05, ∗∗∗ p < 0.001 by Student’s two-tailed t test.

Journal: iScience

Article Title: KIAA1429 promotes clear cell renal cell carcinoma progression by regulating MYC mRNA stability

doi: 10.1016/j.isci.2026.115198

Figure Lengend Snippet: KIAA1429 promoted tumorigenesis of ccRCC cells in vivo (A) Subcutaneous tumor model of ACHN cells with KIAA1429 knockdown. KIAA1429 knockdown led to decreased tumor volume. (B and C) The changes in volume and weight were measured at the indicated weeks after mice were transplanted with the relevant cells. (D) IHC of vimentin, Ki-67, and E-cadherin protein expression in tumors with KIAA1429 knockdown. Data are presented as the means ± SEM. ∗ p < 0.05, ∗∗∗ p < 0.001 by Student’s two-tailed t test.

Article Snippet: KIAA1429 Rabbit Polyclonal antibody , Proteintech , Cat#24761-1-AP.

Techniques: In Vivo, Knockdown, Expressing, Two Tailed Test

MYC might be a potential target for regulation by KIAA1429 in ccRCC (A) KIAA1429 has a significant effect on most RNA-specific processes, cell cycle, cell death, apoptosis, and intracellular signaling pathways, as revealed by GO and KEGG analyses. (B) Heatmap illustrating the downregulated genes in ACHN cells after KIAA1429 knockdown. (C) The expression level of MYC in ccRCC, obtained from the TCGA database. (D) Correlation analysis between the expression of MYC and KIAA1429. Data are presented as the means ± SEM. ∗∗∗ p < 0.001 by Student’s two-tailed t test.

Journal: iScience

Article Title: KIAA1429 promotes clear cell renal cell carcinoma progression by regulating MYC mRNA stability

doi: 10.1016/j.isci.2026.115198

Figure Lengend Snippet: MYC might be a potential target for regulation by KIAA1429 in ccRCC (A) KIAA1429 has a significant effect on most RNA-specific processes, cell cycle, cell death, apoptosis, and intracellular signaling pathways, as revealed by GO and KEGG analyses. (B) Heatmap illustrating the downregulated genes in ACHN cells after KIAA1429 knockdown. (C) The expression level of MYC in ccRCC, obtained from the TCGA database. (D) Correlation analysis between the expression of MYC and KIAA1429. Data are presented as the means ± SEM. ∗∗∗ p < 0.001 by Student’s two-tailed t test.

Article Snippet: KIAA1429 Rabbit Polyclonal antibody , Proteintech , Cat#24761-1-AP.

Techniques: Protein-Protein interactions, Knockdown, Expressing, Two Tailed Test

KIAA1429 regulated the expression of MYC mRNA and its stability in an m6A-dependent manner (A and C) KIAA1429 knockdown inhibits the protein and mRNA expression of MYC in ccRCC cells. (B) KIAA1429 knockdown decreased the stability of MYC mRNA. (D) RIP assay showed that KIAA1429 interacts with MYC mRNA in Caki-1 and ACHN cells. (E) KIAA1429 knockdown reduced overall m6A levels in Caki-1 and ACHN cells. (F) KIAA1429 knockdown reduced m6A levels in MYC. (G) m6A methylation sites on MYC were predicted using the SRAMP database. (H) The pmirGLO-MYC-WT or pmirGLO-MYC-MUT luciferase reporter was transfected into KIAA1429-knockdown and control Caki-1 and ACHN cells, and relative luciferase activity was measured. Data are presented as the ratio of firefly to Renilla luciferase activity. Data are presented as the means ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 by Student’s two-tailed t test.

Journal: iScience

Article Title: KIAA1429 promotes clear cell renal cell carcinoma progression by regulating MYC mRNA stability

doi: 10.1016/j.isci.2026.115198

Figure Lengend Snippet: KIAA1429 regulated the expression of MYC mRNA and its stability in an m6A-dependent manner (A and C) KIAA1429 knockdown inhibits the protein and mRNA expression of MYC in ccRCC cells. (B) KIAA1429 knockdown decreased the stability of MYC mRNA. (D) RIP assay showed that KIAA1429 interacts with MYC mRNA in Caki-1 and ACHN cells. (E) KIAA1429 knockdown reduced overall m6A levels in Caki-1 and ACHN cells. (F) KIAA1429 knockdown reduced m6A levels in MYC. (G) m6A methylation sites on MYC were predicted using the SRAMP database. (H) The pmirGLO-MYC-WT or pmirGLO-MYC-MUT luciferase reporter was transfected into KIAA1429-knockdown and control Caki-1 and ACHN cells, and relative luciferase activity was measured. Data are presented as the ratio of firefly to Renilla luciferase activity. Data are presented as the means ± SEM. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 by Student’s two-tailed t test.

Article Snippet: KIAA1429 Rabbit Polyclonal antibody , Proteintech , Cat#24761-1-AP.

Techniques: Expressing, Knockdown, Methylation, Luciferase, Transfection, Control, Activity Assay, Two Tailed Test

A : Elevated mRNA expression of KIAA1429 in pan-cancer (TCGA database); B : KIAA1429 is highly expressed in CRC tis sues compared to normal tissues (TCGA database, P < 0.001). C : Confirmation of the Figure B results through the GEPIA database( P < 0.05). D : Immunohistochemical staining confirmed higher KIAA1429 expression in CRC tissues compared to adjacent non-tumor tissues (Mann-Whitney U test, P = 2.196e-07); E : High expression of KIAA1429 correlates with poor prognosis (Kaplan-Meier analysis, Log-rank, P = 0.027); F : Nomogram for predicting 1-year survival probability based on clinical factors and KIAA1429 expression. G : Calibration curve assessing nomogram accuracy at 1-year, 3-year, and 5-year time points. H : ROC curve evaluating the diagnostic performance of KIAA1429 expression (AUC = 0.783).

Journal: Scientific Reports

Article Title: KIAA1429 impairs anti-tumor immunity via regulation of PD-L1 and CD8 + T cells in colorectal cancer

doi: 10.1038/s41598-025-27586-6

Figure Lengend Snippet: A : Elevated mRNA expression of KIAA1429 in pan-cancer (TCGA database); B : KIAA1429 is highly expressed in CRC tis sues compared to normal tissues (TCGA database, P < 0.001). C : Confirmation of the Figure B results through the GEPIA database( P < 0.05). D : Immunohistochemical staining confirmed higher KIAA1429 expression in CRC tissues compared to adjacent non-tumor tissues (Mann-Whitney U test, P = 2.196e-07); E : High expression of KIAA1429 correlates with poor prognosis (Kaplan-Meier analysis, Log-rank, P = 0.027); F : Nomogram for predicting 1-year survival probability based on clinical factors and KIAA1429 expression. G : Calibration curve assessing nomogram accuracy at 1-year, 3-year, and 5-year time points. H : ROC curve evaluating the diagnostic performance of KIAA1429 expression (AUC = 0.783).

Article Snippet: Afterwards, primary antibodies, including KIAA1429 (25712-1-AP, Proteintech), PD-L1 (28076-1-AP, Proteintech), and CD8 (66868-1-IG, Proteintech), were applied and incubated overnight at 4 °C.

Techniques: Expressing, Immunohistochemical staining, Staining, MANN-WHITNEY, Diagnostic Assay

A : Correlation of KIAA1429 with tumor immune cell infiltration in CRC. B : CD8 + T cells and Treg cells are highly infiltrated with low KIAA1429 expression in CRC (TCGA database, P < 0.01, P < 0.001). C : Expression of KIAA1429 is negatively correlated with the level of CD8 + T cells infiltration in CRC (TCGA database, r =−0.015, P = 0.001). D : Expression of KIAA1429 is negatively correlated with the level of Treg cells infiltration in CRC (TCGA database, r =−0.233, P < 0.001). E : KIAA1429 was negatively correlated with the infiltration of CD8 + T cells and Tregs in both COAD and READ(TCGA database, P < 0.05, P < 0.01); F : Immunohistochemistry showed reduced CD8 + T-cell infiltration in CRC tissues compared to adjacent normal tissues (Spearman’s correlation analysis, r = 0.0995, P = 0.021).

Journal: Scientific Reports

Article Title: KIAA1429 impairs anti-tumor immunity via regulation of PD-L1 and CD8 + T cells in colorectal cancer

doi: 10.1038/s41598-025-27586-6

Figure Lengend Snippet: A : Correlation of KIAA1429 with tumor immune cell infiltration in CRC. B : CD8 + T cells and Treg cells are highly infiltrated with low KIAA1429 expression in CRC (TCGA database, P < 0.01, P < 0.001). C : Expression of KIAA1429 is negatively correlated with the level of CD8 + T cells infiltration in CRC (TCGA database, r =−0.015, P = 0.001). D : Expression of KIAA1429 is negatively correlated with the level of Treg cells infiltration in CRC (TCGA database, r =−0.233, P < 0.001). E : KIAA1429 was negatively correlated with the infiltration of CD8 + T cells and Tregs in both COAD and READ(TCGA database, P < 0.05, P < 0.01); F : Immunohistochemistry showed reduced CD8 + T-cell infiltration in CRC tissues compared to adjacent normal tissues (Spearman’s correlation analysis, r = 0.0995, P = 0.021).

Techniques: Expressing, Immunohistochemistry

A : Protein-protein interaction (PPI) network of KIAA1429 with RNA-related proteins. B : Gene Ontology (GO) enrichment analysis of KIAA1429-related genes. C : SRAMP database predicts a significant m6A binding site on PD-L1 mRNA; D : KIAA1429 shows a positive correlation with PD-L1 expression in CRC tissues(cBioPortal data, P = 2.275e-03); E , F : Immunohistochemistry validated the positive correlation between KIAA1429 and PD-L1 expression in CRC samples (Spearman correlation analysis, P = 1.017e-07); G : Expression levels of KIAA1429 mRNA in various colorectal cancer cell lines, and KIAA1429 is highly expressed in SW620 cells ( P < 0.001); H : siRNA-mediated silencing of KIAA1429 (siKIAA1429-1,−2,−3), with siKIAA1429-2 exhibiting the most effective knockdown( P < 0.01); I : Reduced PD-L1 mRNA expression following KIAA1429 knockdown in SW620 cells ( P < 0.01); J : Western blot analysis confirmed downregulated PD-L1 protein expression in SW620 cells upon KIAA1429 silencing ( P < 0.01).

Journal: Scientific Reports

Article Title: KIAA1429 impairs anti-tumor immunity via regulation of PD-L1 and CD8 + T cells in colorectal cancer

doi: 10.1038/s41598-025-27586-6

Figure Lengend Snippet: A : Protein-protein interaction (PPI) network of KIAA1429 with RNA-related proteins. B : Gene Ontology (GO) enrichment analysis of KIAA1429-related genes. C : SRAMP database predicts a significant m6A binding site on PD-L1 mRNA; D : KIAA1429 shows a positive correlation with PD-L1 expression in CRC tissues(cBioPortal data, P = 2.275e-03); E , F : Immunohistochemistry validated the positive correlation between KIAA1429 and PD-L1 expression in CRC samples (Spearman correlation analysis, P = 1.017e-07); G : Expression levels of KIAA1429 mRNA in various colorectal cancer cell lines, and KIAA1429 is highly expressed in SW620 cells ( P < 0.001); H : siRNA-mediated silencing of KIAA1429 (siKIAA1429-1,−2,−3), with siKIAA1429-2 exhibiting the most effective knockdown( P < 0.01); I : Reduced PD-L1 mRNA expression following KIAA1429 knockdown in SW620 cells ( P < 0.01); J : Western blot analysis confirmed downregulated PD-L1 protein expression in SW620 cells upon KIAA1429 silencing ( P < 0.01).

Techniques: Binding Assay, Expressing, Immunohistochemistry, Knockdown, Western Blot

A : KIAA1429 was knocked down in MC38 and CT26 cells, and the knockdown efficiency was detected by qPCR. B : Representative tumors images in MC38 and CT26 homozygous mice; C : Body weight(left), tumor volume(middle), and tumor weight(right) in MC38 mice( n = 5); D : Body weight(left), tumor volume(middle), and tumor weight(right) in CT26 mice( n = 5); E : Immunohistochemical staining measures the expression of PD-L1 across different groups.

Journal: Scientific Reports

Article Title: KIAA1429 impairs anti-tumor immunity via regulation of PD-L1 and CD8 + T cells in colorectal cancer

doi: 10.1038/s41598-025-27586-6

Figure Lengend Snippet: A : KIAA1429 was knocked down in MC38 and CT26 cells, and the knockdown efficiency was detected by qPCR. B : Representative tumors images in MC38 and CT26 homozygous mice; C : Body weight(left), tumor volume(middle), and tumor weight(right) in MC38 mice( n = 5); D : Body weight(left), tumor volume(middle), and tumor weight(right) in CT26 mice( n = 5); E : Immunohistochemical staining measures the expression of PD-L1 across different groups.

Techniques: Knockdown, Immunohistochemical staining, Staining, Expressing

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